Inhibition of methionine sulfoxide reduction by dimethyl sulfoxide

Selenium and Methionine Sulfoxide Reduction.

Selenium is an essential trace element because it is present in proteins in the form of selenocysteine residue. Functionally characterized selenoproteins are oxidoreductases. Selenoprotein methionine-R-sulfoxide reductase B1 (MsrB1) is a repair enzyme that reduces ROS-oxidized methionine residues in proteins. Here, we explored a possibility that reversible methionine oxidation is also a mechani...

Reduction of methionine sulfoxide to methionine by Escherichia coli.

L-Methionine-dl-sulfoxide can support the growth of an Escherichia coli methionine auxotroph, suggesting the presence of an enzyme(s) capable of reducing the sulfoxide to methionine. This was verified by showing that a cell-free extract of E. coli catalyzes the conversion of methionine sulfoxide to methionine. This reaction required reduced nicotinamide adenine dinucleotide phosphate and a gene...

Methionine-r-sulfoxide Reductases and Biological Importance of Free Methionine Sulfoxide Reduction

Methionine sulfoxide reduction in mammals: characterization of methionine-R-sulfoxide reductases.

Methionine residues in proteins are susceptible to oxidation by reactive oxygen species, but can be repaired via reduction of the resulting methionine sulfoxides by methionine-S-sulfoxide reductase (MsrA) and methionine-R-sulfoxide reductase (MsrB). However, the identity of all methionine sulfoxide reductases involved, their cellular locations and relative contributions to the overall pathway a...

Stabilization of enveloped viruses by dimethyl sulfoxide.

In a recent review on the storage of animal viruses at subzero temperatures (8), it was pointed out that enveloped viruses present special problems. For example, herpesvirus even at -90 C loses infectivity after prolonged storage and at -20 C is even more labile than at 4 C. Repeated freezings and thawings of this virus result in marked loss in activity. Members of the herpesvirus group can be ...